Transcriptomics, including messenger, micro- and long non-coding RNA sequencing, is an extremely sensitive solution for measuring gene expression.
RNA sequencing provides high-resolution insight into gene expression profiles.
Transcript isoforms, gene fusions, single nucleotide variants can be detected without the need for a targeted RNA sequencing approach.

Sample Requirements

  • Recommended: total RNA, ≥2000 ng, 50 µl, no degradation
  • Minimum: 200 ng, 10 µl

Sequencing Strategy

  • 250-300 bp insert library
  • Paired-end 75 bp sequencing
  • ≥50M sequencing