Transcriptomics, including messenger, micro- and long non-coding RNA sequencing, is an extremely sensitive solution for measuring gene expression.
Sequencing of small RNAs can provide insight into post-transcriptional regulation and gene silencing by detecting expression of isoforms, various microRNAs as well as small non-coding RNA molecules.
Sample Requirements
- Recommended: total RNA, ≥1000 ng, 15 µl, RIN> 7
- Minimum: 1000 ng, 30 µl
Sequencing Strategy
- 40 bp insert library
- Sngle-end 75 bp sequencing
- ≥10Ms sequence