Transcriptomics, including messenger, micro- and long non-coding RNA sequencing, is an extremely sensitive solution for measuring gene expression.

Sequencing of small RNAs can provide insight into post-transcriptional regulation and gene silencing by detecting expression of isoforms, various microRNAs as well as small non-coding RNA molecules.

Sample Requirements

  • Recommended: total RNA, ≥1000 ng, 15 µl, RIN> 7
  • Minimum: 1000 ng, 30 µl

Sequencing Strategy

  • 40 bp insert library
  • Sngle-end 75 bp sequencing
  • ≥10Ms sequence