Transcriptomics, including messenger, micro- and long non-coding RNA sequencing, is an extremely sensitive solution for measuring gene expression.

RNA sequencing provides high-resolution insight into gene expression profiles.

Transcript isoforms, gene fusions, single nucleotide variants can be detected without the need for a targeted RNA sequencing approach.

Sample Requirements

  • Recommended: total RNA, ≥1000 ng, 10 µl, RIN>7
  • Minimum: 200 ng, 10 µl

Sequencing Strategy

  • 250-350 bp insert library
  • Single-end 75 bp sequencing
  • ≥10M sequence