Transcriptomics, including messenger, micro- and long non-coding RNA sequencing, is an extremely sensitive solution for measuring gene expression.
RNA sequencing provides high-resolution insight into gene expression profiles.
Transcript isoforms, gene fusions, single nucleotide variants can be detected without the need for a targeted RNA sequencing approach.
Sample Requirements
- Recommended: total RNA, ≥1000 ng, 10 µl, RIN>7
- Minimum: 200 ng, 10 µl
Sequencing Strategy
- 250-350 bp insert library
- Single-end 75 bp sequencing
- ≥10M sequence