Next-generation sequencing (NGS) methods can be used to determine the DNA sequence of any organism, providing valuable information to answer almost any biological question.

NGS is highly scalable and can be applied to small, targeted regions or to the entire genome. Whole genome sequencing (WGS) is a comprehensive method for analyzing the entire genome of not only humans but any species, such as agriculturally important livestock, plants, or disease-related microbes.

Sample Requirements

  • Recommended: genomic DNA, ≥600 ng, 20 µl, not fragmented
  • Minimum: 200 ng, 15 µl

Sequencing Strategy

  • 250-350 bp insert library
  • Paired-end 150 bp sequencing
  • ≥1000x coverage