Next-generation sequencing (NGS) methods can be used to determine the DNA sequence of any organism, providing valuable information to answer almost any biological question.

NGS is highly scalable and can be applied to small, targeted regions or the entire genome. NGS is highly scalable and can be applied to small, targeted regions or to the entire genome. Whole genome sequencing (WGS) is a comprehensive method for analyzing the entire genome of not only humans but any species, such as agriculturally important livestock, plants or disease-related microbes.

Sample Requirements

  • Recommended: genomic DNA, ≥2000 ng, 50 µl, not fragmented
  • Minimum: 1000 ng, 15 µl

Sequencing Strategy

  • 20-30 Kbp insert library
  • ≥30x coverage